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PNKP
Pathway ID Pathway Name Pathway Description (Reactome) R-HSA-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway. NEIL1 and NEIL2 have a dual DNA glycosylase and beta/delta lyase activity. The AP (apurinic/apyrimidinic) site-directed lyase activity of NEIL1 and NEIL2 is their major physiological role, as they can act on AP sites generated spontaneously or by other DNA glycosylases. NEIL1 or NEIL2 cleave the damaged DNA strand 5' to the AP site, producing a 3' phosphate terminus (3'Pi) and a 5' deoxyribose phosphate terminus (5'dRP). DNA polymerase beta (POLB) excises 5'dRP residue but is unable to add the replacement nucleotide to DNA with the 3'Pi end. PNKP, a DNA 3' phosphatase, removes 3'Pi and enables POLB to incorporate the replacement nucleotide, which is followed by ligation of repaired DNA strand by XRCC1:LIG3 complex (Whitehouse et al. 2001, Wiederhold et al. 2004, Das et al. 2006)
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source APP Reconstituted Complex physical 21832049 , (Europe PMC )NA BioGRID ATAD3B Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID ATXN3 Co-localization, PCA physical 25590633 , (Europe PMC )NA BioGRID C18orf25 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID CCDC43 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID CDK11B Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID DCAF1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID DCPS Co-fractionation physical 26344197 , (Europe PMC )NA BioGRID DDX20 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct DNAAF5 Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID EED Affinity Capture-MS physical 24457600 , (Europe PMC )NA BioGRID FOXO3 Affinity Capture-MS physical 25609649 , (Europe PMC )NA BioGRID GEMIN4 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID GZMK Two-hybrid, two hybrid array physical, physical association 21988832 , (Europe PMC )0.37 BioGRID, IntAct HBA2 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID HBB Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID HNRNPU Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID IKZF1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.49, 0.56 BioGRID, IntAct KDM1A Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT KRTAP4-12 Two-hybrid, two hybrid pooling approach physical, physical association 16189514 , (Europe PMC )0.37 BioGRID, IntAct LAS1L Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct LIG3 Affinity Capture-MS, Affinity Capture-Western, Two-hybrid, anti bait coimmunoprecipitation physical, physical association 11163244 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct LIG4 Affinity Capture-MS, Affinity Capture-Western, anti bait coimmunoprecipitation physical, physical association 15385968 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct LMNA Two-hybrid physical 24623722 , (Europe PMC )NA BioGRID MAGEA11 Two-hybrid, two hybrid pooling approach physical, physical association 16189514 , (Europe PMC )0.37 BioGRID, IntAct MCM3 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 15385968 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct MCM5 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID MID1 Co-fractionation physical 22939629 , (Europe PMC )NA BioGRID MYOZ1 Two-hybrid physical 16189514 , (Europe PMC )NA BioGRID NASP Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID NF2 Affinity Capture-MS physical 26549023 , (Europe PMC )NA BioGRID NOL3 Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID NOLC1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID NOM1 Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID NRDC Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID NTRK1 Affinity Capture-MS physical 25921289 , (Europe PMC )NA BioGRID NUP93 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct PARP1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID POLB Affinity Capture-MS, Affinity Capture-Western, Two-hybrid physical 11163244 , 26186194 , 28514442 , (Europe PMC )NA BioGRID PRKDC Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID RPA1 Affinity Capture-MS physical 24332808 , (Europe PMC )NA BioGRID RPA2 Affinity Capture-MS physical 24332808 , (Europe PMC )NA BioGRID RPA3 Affinity Capture-MS physical 24332808 , (Europe PMC )NA BioGRID SBDS Co-fractionation physical 26344197 , (Europe PMC )NA BioGRID SLC25A12 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct SMAD5 Two-hybrid, two hybrid physical, physical association 15231748 , (Europe PMC )0.37 BioGRID, IntAct, MINT SSRP1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID SUPT16H Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID SUV39H1 Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT TCOF1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID TNIP1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct TRIM25 Affinity Capture-RNA physical 29117863 , (Europe PMC )NA BioGRID TRIM26 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID TRIM37 Two-hybrid physical 16189514 , (Europe PMC )NA BioGRID UBE2O Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID XRCC1 Affinity Capture-MS, Affinity Capture-Western, Far Western, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, isothermal titration calorimetry, pull down, x-ray crystallography association, direct interaction, physical, physical association 11163244 , 15367657 , 15385968 , 17353931 , 19155274 , 21637298 , 26496610 , (Europe PMC )0.83 BioGRID, IntAct, MINT XRCC4 Affinity Capture-MS, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, experimental interaction detection, isothermal titration calorimetry, pull down, two hybrid array, two hybrid prey pooling approach association, physical, physical association 15385968 , 17353931 , 21637298 , 26186194 , 28514442 , Europe PMC )0.84 BioGRID, IntAct, MINT XRCC5 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID XRCC6 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID YWHAQ Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct ZBTB14 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct ZNF639 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source DNAAF5 {ECO:0000303|PubMed:25232951, anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct EBI-1059129 anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct EBI-1059318 anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct FOXO3 pull down, tandem affinity purification association, physical association 25609649 , (Europe PMC )0.50 IntAct, MINT GZMK Two-hybrid, two hybrid array physical, physical association 21988832 , (Europe PMC )0.37 BioGRID, IntAct IKZF1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.49, 0.56 BioGRID, IntAct KDM1A Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT KRTAP4-12 Two-hybrid, two hybrid pooling approach physical, physical association 16189514 , (Europe PMC )0.37 BioGRID, IntAct LAS1L Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct LIG3 Affinity Capture-MS, Affinity Capture-Western, Two-hybrid, anti bait coimmunoprecipitation physical, physical association 11163244 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct LIG4 Affinity Capture-MS, Affinity Capture-Western, anti bait coimmunoprecipitation physical, physical association 15385968 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct MAGEA11 Two-hybrid, two hybrid pooling approach physical, physical association 16189514 , (Europe PMC )0.37 BioGRID, IntAct MCM3 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 15385968 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct MYOZ1 two hybrid pooling approach physical association 16189514 , (Europe PMC )0.37 IntAct NOL3 anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct NUP93 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct SLC25A12 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct SMAD5 Two-hybrid, two hybrid physical, physical association 15231748 , (Europe PMC )0.37 BioGRID, IntAct, MINT SUV39H1 Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT TNIP1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct TP53BP1 fluorescence microscopy colocalization 21637298 , (Europe PMC )0.27 IntAct, MINT TRIM37 two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach physical association 16189514 , Europe PMC )0.62 IntAct XRCC1 Affinity Capture-MS, Affinity Capture-Western, Far Western, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, isothermal titration calorimetry, pull down, x-ray crystallography association, direct interaction, physical, physical association 11163244 , 15367657 , 15385968 , 17353931 , 19155274 , 21637298 , 26496610 , (Europe PMC )0.83 BioGRID, IntAct, MINT XRCC4 Affinity Capture-MS, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, experimental interaction detection, isothermal titration calorimetry, pull down, two hybrid array, two hybrid prey pooling approach association, physical, physical association 15385968 , 17353931 , 21637298 , 26186194 , 28514442 , Europe PMC )0.84 BioGRID, IntAct, MINT YWHAQ Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct YWHAZ anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct ZBTB14 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct ZNF639 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source KDM1A Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT SMAD5 Two-hybrid, two hybrid physical, physical association 15231748 , (Europe PMC )0.37 BioGRID, IntAct, MINT SUV39H1 Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT TP53BP1 fluorescence microscopy colocalization 21637298 , (Europe PMC )0.27 IntAct, MINT XRCC1 Affinity Capture-MS, Affinity Capture-Western, Far Western, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, isothermal titration calorimetry, pull down, x-ray crystallography association, direct interaction, physical, physical association 11163244 , 15367657 , 15385968 , 17353931 , 19155274 , 21637298 , 26496610 , (Europe PMC )0.83 BioGRID, IntAct, MINT XRCC4 Affinity Capture-MS, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, experimental interaction detection, isothermal titration calorimetry, pull down, two hybrid array, two hybrid prey pooling approach association, physical, physical association 15385968 , 17353931 , 21637298 , 26186194 , 28514442 , Europe PMC )0.84 BioGRID, IntAct, MINT
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Interacting partner Detection method Interaction type PubMed IDs Interaction Score Source APP Reconstituted Complex physical 21832049 , (Europe PMC )NA BioGRID ATAD3B Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID ATXN3 Co-localization, PCA physical 25590633 , (Europe PMC )NA BioGRID C18orf25 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID CCDC43 Affinity Capture-MS physical 26186194 , 28514442 , (Europe PMC )NA BioGRID CDK11B Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID DCAF1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID DCPS Co-fractionation physical 26344197 , (Europe PMC )NA BioGRID DDX20 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct DNAAF5 Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID DNAAF5 {ECO:0000303|PubMed:25232951, anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct EBI-1059129 anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct EBI-1059318 anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct EED Affinity Capture-MS physical 24457600 , (Europe PMC )NA BioGRID FOXO3 Affinity Capture-MS physical 25609649 , (Europe PMC )NA BioGRID FOXO3 pull down, tandem affinity purification association, physical association 25609649 , (Europe PMC )0.50 IntAct, MINT GEMIN4 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID GZMK Two-hybrid, two hybrid array physical, physical association 21988832 , (Europe PMC )0.37 BioGRID, IntAct HBA2 Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID HBB Affinity Capture-MS physical 28514442 , (Europe PMC )NA BioGRID HNRNPU Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID IKZF1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.49, 0.56 BioGRID, IntAct KDM1A Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT KRTAP4-12 Two-hybrid, two hybrid pooling approach physical, physical association 16189514 , (Europe PMC )0.37 BioGRID, IntAct LAS1L Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct LIG3 Affinity Capture-MS, Affinity Capture-Western, Two-hybrid, anti bait coimmunoprecipitation physical, physical association 11163244 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct LIG4 Affinity Capture-MS, Affinity Capture-Western, anti bait coimmunoprecipitation physical, physical association 15385968 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct LMNA Two-hybrid physical 24623722 , (Europe PMC )NA BioGRID MAGEA11 Two-hybrid, two hybrid pooling approach physical, physical association 16189514 , (Europe PMC )0.37 BioGRID, IntAct MCM3 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 15385968 , 17353931 , (Europe PMC )0.40 BioGRID, IntAct MCM5 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID MID1 Co-fractionation physical 22939629 , (Europe PMC )NA BioGRID MYOZ1 Two-hybrid physical 16189514 , (Europe PMC )NA BioGRID MYOZ1 two hybrid pooling approach physical association 16189514 , (Europe PMC )0.37 IntAct NASP Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID NF2 Affinity Capture-MS physical 26549023 , (Europe PMC )NA BioGRID NOL3 Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID NOL3 anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct NOLC1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID NOM1 Affinity Capture-MS physical 17353931 , (Europe PMC )NA BioGRID NRDC Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID NTRK1 Affinity Capture-MS physical 25921289 , (Europe PMC )NA BioGRID NUP93 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct PARP1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID POLB Affinity Capture-MS, Affinity Capture-Western, Two-hybrid physical 11163244 , 26186194 , 28514442 , (Europe PMC )NA BioGRID PRKDC Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID RPA1 Affinity Capture-MS physical 24332808 , (Europe PMC )NA BioGRID RPA2 Affinity Capture-MS physical 24332808 , (Europe PMC )NA BioGRID RPA3 Affinity Capture-MS physical 24332808 , (Europe PMC )NA BioGRID SBDS Co-fractionation physical 26344197 , (Europe PMC )NA BioGRID SLC25A12 Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct SMAD5 Two-hybrid, two hybrid physical, physical association 15231748 , (Europe PMC )0.37 BioGRID, IntAct, MINT SSRP1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID SUPT16H Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID SUV39H1 Two-hybrid, two hybrid physical, physical association 23455924 , (Europe PMC )0.37 BioGRID, IntAct, MINT TCOF1 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID TNIP1 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct TP53BP1 fluorescence microscopy colocalization 21637298 , (Europe PMC )0.27 IntAct, MINT TRIM25 Affinity Capture-RNA physical 29117863 , (Europe PMC )NA BioGRID TRIM26 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID TRIM37 Two-hybrid physical 16189514 , (Europe PMC )NA BioGRID TRIM37 two hybrid array, two hybrid pooling approach, two hybrid prey pooling approach physical association 16189514 , Europe PMC )0.62 IntAct UBE2O Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID XRCC1 Affinity Capture-MS, Affinity Capture-Western, Far Western, Two-hybrid, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, isothermal titration calorimetry, pull down, x-ray crystallography association, direct interaction, physical, physical association 11163244 , 15367657 , 15385968 , 17353931 , 19155274 , 21637298 , 26496610 , (Europe PMC )0.83 BioGRID, IntAct, MINT XRCC4 Affinity Capture-MS, Reconstituted Complex, anti bait coimmunoprecipitation, anti tag coimmunoprecipitation, experimental interaction detection, isothermal titration calorimetry, pull down, two hybrid array, two hybrid prey pooling approach association, physical, physical association 15385968 , 17353931 , 21637298 , 26186194 , 28514442 , Europe PMC )0.84 BioGRID, IntAct, MINT XRCC5 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID XRCC6 Affinity Capture-MS physical 15385968 , (Europe PMC )NA BioGRID YWHAQ Affinity Capture-MS, anti bait coimmunoprecipitation physical, physical association 17353931 , (Europe PMC )0.40 BioGRID, IntAct YWHAZ anti bait coimmunoprecipitation physical association 17353931 , (Europe PMC )0.40 IntAct ZBTB14 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct ZNF639 Two-hybrid, two hybrid array, two hybrid prey pooling approach, validated two hybrid physical, physical association 25416956 , Europe PMC )0.70 BioGRID, IntAct
Phosphorylating Kinases Phosphoresidues Detection Method PubMed IDs Source
Visualize a network of protein kinases and phosphatases phosphorylating and dephosphorylating this protein respectively
ATM S114_EETRTPEsQPDTPPG , S126_PPGTPLVsQDEKRDA , NA NA PhosphoSitePlus , Unknown S114_EETRTPEsQPDTPPG , S126_PPGTPLVsQDEKRDA , T111_LRWEETRtPESQPDT , T118_TPESQPDtPPGTPLV , T122_QPDTPPGtPLVSQDE , HTP, in vivo 17081983 , 17525332 , 18077418 , 18452278 , 18669648 , 19413330 , 19651622 , 19664994 , 19664995 , 20058876 , 20068231 , 20166139 ,(Europe PMC )HPRD, PhosphoELM ,
Statistics
